Knowledge Base

Explore the different cutting modes on the Leica RM2255 Microtome in this video, and learn how to optimize your sectioning for maximum precision and efficiency!

Need to set the time on your AVANTIK QS11, Microm HM 520, or HM 525 Cryostat? Watch this quick guide to ensure accurate timing for precise cryosectioning every time!

Learn how to properly set the defrost function on the AVANTIK QS11, Microm HM 520, and HM 525 Cryostats to keep your equipment running smoothly and efficiently!

Struggling with cryostat temperature settings? Watch this video to master chamber temp and Peltier adjustments on the AVANTIK QS11, Microm HM 520, and HM 525 Cryostats for optimal sectioning results!

See the AVANTIK QS11, Microm HM 520, and HM 525 Cryostats in action as we break down their sectioning and trimming modes for precise frozen tissue samples!

In this video, we will show you the basic controls and features used when programming the LEFT SIDE CONTROL PANEL of an AVANTIK QS11 CRYOSTAT.

A standard H&E staining protocol is provided below.  It applies to either a manual or automated staining procedure.  While it may not be ideal for your laboratory, it can be used as a starting point.  The final color rendition of your H&E stain should be determined by working with your pathologists.  This will consequently make their job easier.  Each day, once the H&E stain set up is completed, you should run down one test slide to confirm that the staining is optimal.  This also will help you to document quality control procedures.

What exactly is a routine “H&E”?  And what makes it routine?  The first question is easy.  “H” stands for ”hematoxylin” and “E” stands for “eosin”.  Both are dyes used to stain tissue sections in histology.  However, the procedure for correctly applying this combination of stains to tissue sections is far from routine.

In the histology world, the mere mention of a “silver stain” may be the cause of panic and uncertainty with regard to the performance of the stain, and the quality of the final resulting microscope slide.  All other special stains, with few exceptions, are relatively easy and straightforward to perform; not so with silver stains.

The staining of microorganisms in histology can be challenging. Filamentous fungi and associated conidia are more easily demonstrated as they are visible under light microscopy when stained with periodic acid Schiff’s (PAS).

Fungi include molds, yeasts and higher fungi. All fungi are eukaryotic and have sterols but not peptidoglycan in their cell membrane. Their cell walls are composed of cellulose; the same building blocks that plants use.